Monoadduct forming photochemical reagents for labeling nucleic acids for hybridization
نویسندگان
چکیده
منابع مشابه
Monoadduct forming photochemical reagents for labeling nucleic acids for hybridization.
We describe the synthesis of three angelicin derivatives which can be used for labeling nucleic acids with biotin. These compounds were used to label nucleic acids in the presence of lysed cell constituents. The resulting labelled nucleic acids show hybridization to a genus specific probe for E. coli. The relative comparison of sensitivity indicates that a polyamine linker is better than a poly...
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Electron paramagnetic resonance (EPR) spectroscopy has emerged as a valuable technique to study the structure and dynamics of nucleic acids and their complexes with other biomolecules. EPR studies require incorporation of stable free radicals (spin labels), usually aminoxyl radicals (nitroxides), at specific sites in the nucleic acids using site-directed spin labeling (SDSL). In addition to the...
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Several nucleic acids hybridization-based approaches, such as microarray, competitive genomic, and Southern or Northern blot hybridization, have become popular tools for specialists in biochemistry and in biomedicine, and are now in routine use. However, the potential of in-solution nucleic acids hybridization-based experimental techniques seems to be underestimated now. Examples are subtractiv...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1989
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/17.11.4293